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Gupta, P. K.
- Evergreen Revolution in Agriculture: Pathway to a Green Economy
Abstract Views :234 |
PDF Views:76
Authors
Affiliations
1 Molecular Biology Laboratory, Department of Genetics and Plant Breeding, CCS University, Meerut 250 004, IN
1 Molecular Biology Laboratory, Department of Genetics and Plant Breeding, CCS University, Meerut 250 004, IN
Source
Current Science, Vol 106, No 1 (2014), Pagination: 104-105Abstract
No Abstract.- Competing Endogenous RNA (ceRNA): a New Class of RNA Working as Mirna Sponges
Abstract Views :247 |
PDF Views:96
Authors
Affiliations
1 Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut 250 004, IN
1 Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut 250 004, IN
Source
Current Science, Vol 106, No 6 (2014), Pagination: 823-830Abstract
MicroRNAs (miRNAs) are widely known as a class of non-coding RNA (ncRNA), which regulate gene expression, largely at the post-transcriptional level, but rarely also at the transcription level. During the last few years, a new class of ncRNA has been discovered, which counteracts the repressive activity of miRNA, by sequestering miRNA within a cell. These novel ncRNA molecules have been variously described as 'miRNA sponges/decoys', 'target mimics' or 'competing endogenous RNA'. Several reports of the occurrence of these ceRNAs and their role in regulating gene expression were published recently, which are briefly described in this article. These discoveries will keep scientists busy for many years to generate new information.Keywords
Competing Endogenous RNA, Eukaryotic Genome, Gene Expression, miRNA Sponges.- Synthetic Genomics: From Synthesis of Prokaryotic Genomes to Synthesis of a Fully Functional Eukaryotic Chromosome
Abstract Views :305 |
PDF Views:78
Authors
P. K. Gupta
1,
V. Jaiswal
1
Affiliations
1 Department of Genetics and Plant Breeding, CCS University, Meerut 250 004, IN
1 Department of Genetics and Plant Breeding, CCS University, Meerut 250 004, IN
Source
Current Science, Vol 107, No 12 (2014), Pagination: 1975-1982Abstract
Synthetic genomics is a new area of research and had its origin with the report of synthesis of the gene for yeast alanyl tRNA by Khorana and his co-workers in 1970. This was followed by the synthesis of a number of protein coding genes during 1980s and thereafter. In 1996, the concept of a minimal genome was put forward, suggesting that the synthesis of a genome by eliminating all nonessential genes should be possible and needs to be tried. Synthesis of genomes of some viruses like poliovirus and PhiX174, and those of bacteria including Mycoplasma genitalium and M. mycoides was reported during the first decade of the present century. In parallel with these, efforts underway at JC Venter Institute (JCVI), a project called Sc2.0 for the synthesis of all the 16 chromosomes of yeast, took shape at John Hopkins University in USA under the leadership of Jef Boeke and S. Chandrasegaran. Under this project, the synthesis of two chromosome arms (VIL and IXR) was reported in 2011 and that of the first functional chromosome (SynIII) was reported in April 2014, which marked the beginning of a new era in the field of synthetic genomics. Many more eukaryotic chromosomes will be synthesized in future and will be utilized for a variety of purposes.Keywords
Eukaryotic Chromosome, Prokaryotic Genomes, Synthetic Biology, Synthetic Genomics.- Development of Hydrophobic Platinum-Doped Carbon Aerogel Catalyst for Hydrogen-Deuterium Exchange Process at High Pressure
Abstract Views :204 |
PDF Views:88
Authors
Rashmi Singh
1,
M. K. Singh
1,
D. K. Kohli
1,
Ashish Singh
1,
Sushmita Bhartiya
2,
A. K. Agarwal
3,
P. K. Gupta
1
Affiliations
1 Nano Functional Materials Laboratory, Laser Materials Development and Devices Division, Raja Ramanna Centre for Advanced Technology, Indore 452 013, IN
2 Nano Functional Materials Laboratory, Laser Materials Development and Devices Division, Raja Ramanna Centre for Advanced Technology, Indore 452 013, IS
3 Heavy Water Board, Vikram Bhawan, Anushakti Nagar, Mumbai 400 094, IN
1 Nano Functional Materials Laboratory, Laser Materials Development and Devices Division, Raja Ramanna Centre for Advanced Technology, Indore 452 013, IN
2 Nano Functional Materials Laboratory, Laser Materials Development and Devices Division, Raja Ramanna Centre for Advanced Technology, Indore 452 013, IS
3 Heavy Water Board, Vikram Bhawan, Anushakti Nagar, Mumbai 400 094, IN
Source
Current Science, Vol 109, No 10 (2015), Pagination: 1860-1864Abstract
The use of catalysed exchange of deuterium (D) between hydrogen (H) gas and liquid water using the bithermal hydrogen water (BHW) process is a promising and environment-friendly approach for the production of heavy water. However, the use of this approach is limited by the lack of a suitable catalyst that has good activity at high operating pressures required for practical applications. We report the development of hydrophobic platinum-doped carbon aerogel (PtCA) catalyst which shows good catalytic activity for H/D isotope exchange reactions at operating pressures up to 20 bar.Keywords
Carbon Dioxide Activation, Hydrogen Isotope Separation, Hydrophobic Catalyst, Platinum-Doped Carbon Aerogel.- Food and Nutritional Security
Abstract Views :244 |
PDF Views:74
Authors
Affiliations
1 Department of Genetics and Plant Breeding, CCS University, Meerut-250 004, IN
1 Department of Genetics and Plant Breeding, CCS University, Meerut-250 004, IN
Source
Current Science, Vol 113, No 05 (2017), Pagination: 835-835Abstract
I have read with interest the Guest Editorial entitled 'Food for all in anthropocene era' by M. S. Swaminathan (MSS) and P. C. Kesavan (PCK). MSS is known to many of us as a world leader in the field of agriculture, and we have always admired him and derived inspiration from the way he provided this leadership.References
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- Swaminathan, M. S., Curr. Sci., 2016, 111, 965.
- T-Cells and Cancer Immunotherapy (Immuno-Oncology):The 2018 Nobel Prize in Physiology or Medicine
Abstract Views :246 |
PDF Views:74
Authors
Affiliations
1 Department of Genetics and Plant Breeding, CCS University, Meerut 250 004, IN
1 Department of Genetics and Plant Breeding, CCS University, Meerut 250 004, IN
Source
Current Science, Vol 115, No 9 (2018), Pagination: 1631-1635Abstract
The 2018 Nobel Prize in Physiology or Medicine has been awarded to two scientists, James P. Allison and Tasuku Honjo for discovery of checkpoint molecules CTLA-4 and PD-1, which inhibit the T-cells of the im-mune system from attacking the cancerous tumours. They also discovered antibodies, which could be used for inhibiting the activity of these checkpoint molecules, thus relieving the T-cells from inhibitory activity of checkpoints, to be able to attack cancerous tumours. Such antibodies have already been converted into a number of immunotherapeutic drugs, which have been approved and are already being used for treatment of a variety of cancers.References
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- Beyond Crispr:Single Base Editors for Human Health and Crop Improvement
Abstract Views :250 |
PDF Views:75
Authors
Affiliations
1 Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut - 250 004, IN
1 Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut - 250 004, IN
Source
Current Science, Vol 116, No 3 (2019), Pagination: 386-397Abstract
During 2016–2018, CRISPR/Cas9 technology was modified using disabled Cas9 with nickase activity in combination with cytosine/adenine deaminases for the development of four generations of cytosine base editors (BE1–BE4) for C → U conversion and at least seven generations of adenine base editors (ABE1–ABE7) for A → I conversion. These base editors exhibited improved efficiency and reduced frequency of deletions among the products. Further improvement in the form of enhanced base editors and high-fidelity base editors was achieved through the use of 1-3 copies of uracil N-glycosylase inhibitors and phage Mu-Gam protein. The technology will bring precision to gene editing technology for human healthcare and crop improvement.Keywords
AID/APOBEC, Base Editing, CRISPR/Cas9, Cytidine/Adenine Deaminases, Target AID.References
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- Saey, T. H., New CRISPR gene editors can fix RNA and DNA one typo at a time. Sci. News, 25 October 2017.
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- Komor, A. C. et al., Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci. Adv., 2017, 3, eaao4774.
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- Scatterometry for Land Hydrology Science and its Applications
Abstract Views :253 |
PDF Views:66
Authors
Affiliations
1 Earth, Ocean, Atmosphere, Planetary Sciences and Applications Area (EPSA), Space Applications Centre, ISRO, Ahmedabad 380 015, IN
1 Earth, Ocean, Atmosphere, Planetary Sciences and Applications Area (EPSA), Space Applications Centre, ISRO, Ahmedabad 380 015, IN
Source
Current Science, Vol 117, No 6 (2019), Pagination: 1014-1021Abstract
This study reports the potential of SCATSAT-1 scatterometer data for catchment-scale hydrological applications related with river water level estimation and flood detection. New approaches have been developed for estimation of river water levels and detection of surface flooding using Oceansat-II scatterometer (OSCAT) and SCATSAT-1 scatterometer-based highresolution backscatter and brightness temperature (BT) datasets respectively. Ku-band sigma-0 and BT data, Shuttle Radar Topography Mission Digital Elevation Model and observed hydrometric data have been used in this study. Catchments of gauging sites and their influencing areas were delineated using the topography, wetness conditions and land-cover variations. OSCAT time series of scatterometer image reconstruction data were used to develop model function between basin water index and ground-observed river-stage datasets. Subsequently, inverting these functions on SCATSAT-1 observations, river water levels for 2017 were estimated at different gauging sites. A study on the magnitude of each flooding event in terms of intensity, duration and extent of area affected was also carried out using the scatterometerbased BT data analysis. The study demonstrated that high temporal resolution scatterometer data has the potential to fill the gap of coarser temporal resolution altimeters (10–35 days) for river heights and Synthetic Aperture Radar Data (7–25 days) for surface flooding with the advantage of capturing extreme events.Keywords
Backscattering Coefficient, Brightness Temperature, River Water Level, Scatterometers, Soil Wetness.References
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